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IDH1 Antibody produced by Dianova

17 January 2012

The PathWest Neuropathology Laboratory at Royal Perth Hospital currently uses the anti-human IDH1 R132H antibody (DIA H09 100 micrograms) at a concentration of 0.2 milligrams/ml, clone H09 on the Ventana with successful results.  Our Ventana protocol is a dilution of 1 in 100 and with CC1 mild antigen retrieval at room temperature with 32 minutes incubation and no amplification. 

At the last AGM  in June 2011 Australian laboratories have a number of different successful protocols for this antibody. Our results are confirmed by molecular testing which also excludes false negative results.

 

If you wish to share your protocols please Contact Us to place the information on this Forum.